Allelopathic effect of calotropis procera on growth and antioxidant activity of brassica. Seeds of brassica were soaked in solutions containing 20%, 40%, 60% and 80% concentrations of leaf, fruit and flower extract of c. Analysis of antioxidant activity in extracts of calotropis procera ait. Antioxidant, calotropis procera, cytotoxicity, polyphenol. The present study revealed some endophytic fungi from calotropis procera could be a potential source of novel natural antioxidant compounds. Diabetes mellitus is a disease that affects about 10% of the population. Antimicrobial activities of calotropis procera on selected. Phytochemical analysis, antibacterial and antioxidant. Antioxidant, calotropis procera, free radicals, wistar rats. Ramesh joshi, anshul sharma and bhanwar lal jat plant biotechnology laboratory, department of botany, government college, kesar ganj, beawar road, ajmer 305001india. Calotropis gigantea, flowers, gcms analysis, antioxidant.
Analysis of antioxidant activity in different extracts of calotropis. Hepatoprotective and antioxidant activity of root bark of calotropis procera r. The cytotoxic potential of stem organic extracts from calotropis procera asclepiadaceae was firstly evaluated against cancer cell lines by mtt assay. Antioxidant activity and mineral element contents of calotropis. Pdf antioxidant activity of the leaf extracts of calotropis. Antiedematogenic and antioxidant properties of high molecular weight protein subfraction of calotropis procera latex in rat abstract objectives. Phytochemistry and ethnopharmaceutics of calotropis procera. Calotropis procera extracts agrees with the work of nenaah et al. The anti oxidant activity in different extracts and fractions of the leaves viz. Comparative analysis of antioxidant properties of extracts of. Aiton,apocynaceae, popularly known as algodaodeseda, is a wild african bush, rich in bioactive substances that determine the medicinal potential of this species. Aspergillus and fusarium genus species showed significant antioxidant activity by the different assay. The plant is erect, tall, large, much branched and perennial with milky latex throughout.
Calotropis procera is small, erect shrub, which is used in several herbal and empirical medicines to cure simple and deadly diseases and disorders. Antiinflammatory and analgesic activities of ethanolic. Antioxidant, cytotoxicity and polyphenolic content of. The root and latex of calotropis gigantea were screened for its antimicrobial and phytochemical activities. Calotropis procera are widely used traditional medicinal plant to treat various ailments. Antimicrobial activity and phytochemical analysis of. Antioxidant activity, total phenolic and total flavonoid contents of stem back and root methanolic extracts of calotropis procera wilton mbinda, colleta musangi abstract calotropis procera it is known for traditional kenyan medicinal system and it is used for it has previously been employed for treatment various diseases. Phytochemical investigation of different plant parts of calotropis procera a. Subsequently, samples considered cytotoxic were tested for antimitotic activity on sea urchin egg development and for in vivo antiproliferative activity in mice bearing sarcoma 180 tumor. Calotropis procera belongs to the family asdepiadaceae, which has been used as a folk medicine. Pdf in vitro antioxidant activity of calotropis procera.
The antibacterial activity of 16 plant latexes was screened. The antioxidant potential of the methanolic extract was determined on the basis of their scavenging activity of the stable 1,1diphenyl2picryl hydrazyl dpph free radical. The leaves of calotropis procera were air dried, grounded. Phytochemical and pharmacological studies of the whole plant. Antiedematogenic and antioxidant properties of high. Pdf in vitro antioxidant activity of calotropis procera researchgate. Taking ascorbic acid as the standard, the absorbance of each of the solutions were determined at 517nm using double beam uvvisible spectrophotometer rayleigh uv2601.
Furthermore, the same extract showed appreciable antiinflammatory via lipoxygenase inhibitory activity lox, ic 50 values ranged from 7. Although there are some reports showing the plant effective in folk use but there were no scientific reports for antioxidant activity of petroleum ether extract. Total phenolic content and in vitro antioxidant activity. Antioxidant, antimicrobial activities and gcms analysis of calotropis gigantea white flowers shopna rajamohan, prabakaran kalaivanan, ilayaraja sivagnanam, manivannan rajamanickam abstract calotropis gigantea white asclepiadaceae, is a weed plant commonly known as giant milkweed. The communication deals with the antioxidant activity of aqueous leaf extract of calotropis procera ait. With this background, the antioxidant properties of aqueous leaf extract of calotropis procera along with its phytochemical, pharmacological and nutritive. Anticancer and antibacterial activity of calotropis.
The antioxidant activity in different extracts and fractions of the leaves viz. Antioxidant activity of root of calotropis procera extract was evaluated in a series of in vitro assay involving free radicals. Result of the antimicrobial activity of root extracts of calotropis gigantea. Analysis of antioxidant activity in different extracts of. Anthelmintic activity the anthelmintic activity of calotropis procera linn. Endophytic fungi are microorganisms that colonize inside the plant tissues without causing any disease symptoms. Total phenolic content and in vitro antioxidant activity of. Calotropis procera, dpph free radical, nitric oxide radical. Hence, it is distributed in tropical and subtropical. The antioxidant study was evaluated in vitro, using 2,2diphenylpicrylhydrazyl dpph and deoxyribose degradation assays. Shrivastava, suchita singh, sanchita singh department of botany and biotechnology govt. The calotropis gigantia latex had a completely b asic. Antioxidant activity, calotropis procera, dpph assay, endophytic fungi, total phenolic content, xanthine oxidase inhibitory assay.
The present study was carried out to evaluate the antioxidant and antibacterial activity of leaves extracts of calotropis procera and calotropis gigantea and characterization of their bioactive metabolites by fourier transform infrared ftir spectroscopy and gas chromatographymass spectroscopy gcms. Phytochemical and pharmacological studies of the whole. Calotropis procera is also reported widely in various folklore preperations and ethnomedicines. It was thus deemed of interest to investigate and compare the antiulcer and cytotoxic activities of c. Phytochemical investigation of different plant parts of. Phytochemical and antibacterial activities of water, methanol and ethanol extracts obtained from the fruit and bark of calotropis procera were investigated in an attempt to evaluate its medicinal potentials. Antibacterial activity of crude extract and latex crude was 20. Antiinflammatory and analgesic activities of ethanolic leaf extract of calotropis procera 1saba a. Antibacterial and antioxidant activity of plant latex. Hepatoprotective activity of the methanol extract mcp and phytoconstituents directed three sub fractions hexane hcp, ethylacetate ecp and chloroform ccp of the root bark was determined using carbon tetrachloride ccl 4 induced liver injury in mice.
Antioxidant and cytotoxic flavonols from calotropis procera. The phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, and cardiac glycosides with a very high content in water extracts. Biological morphology and ethanopharmocological importance. From the results it was evident that the flower of vinca rosea showed the highest antioxidant activity of 97. Hepatoprotective activity of calotropis procera flowers against paracetamolinduced hepatic injury in rats. Endophytic fungi isolated from medicinal plants have emerged as an interesting source for the isolation of bioactive compounds. Phytochemicals screening and free radical scavenging potential of. Calotropis procera, endophytic fungi, colonisation frequency, green synthesised agnps. However the aqueous extract showed mild antioxidant activity. The invitro antioxidant activity of root was investigated by dpph and frap method in both method, plant extract possess high antioxidant activity. College, patna, magadh university, bihar, india corresponding author a b s t r a c t keywords calotropis e pharmacology, m. Pdf allelopathic effect of calotropis procera on growth. Similarly antioxidant activity of leaf extract of c.
The remarkable antidiarrheal activity of calotropis procera leaves extract against castoroil induced diarrhea model proved its utility in a good rang of diarrheal cases 11. Antioxidant potential of a toxic plant calotropis procera. Calotropis procera is traditionally used for treating many diseases including ulcers and tumors. Calotropis procera is a medicinal plant used traditionally in animal and human medicine for its digestive and antiasthmatic effects.
Antioxidant and antibacterial activities of calotropis. Phytochemical and antibacterial properties of calotropis. The total antioxidant activity was not very pronounced, however, the reducing potential of the extract was prominent, and showed concentration dependence. In the present study, antioxidant activity and mineral element contents of the leaves and flowers of c. Flowers, in comparison with levamisole, was evaluated in a series of in vitro and in vivo studies. Rizgallah 3 1department of biological sciences, faculty of science, king abdulaziz university, p. A study on phytochemical screening and invitro antioxidant. Antioxidant activity, total phenolic and total flavonoid.
Identification of pcoumaric and phydroxybenzoic acids in adhatoda vasica and calotropis procera. Studies on endophytic fungi of calotropis procera l. Antioxidant activity, lipoxygenase inhibitory effect and. Antiedematogenic and antioxidant properties of high molecular. Phytochemicals were analysed qualitatively and the result confirmed the presence of alkaloids, phenols, saponin, steroids. In the present study, calotropis procera asclepediaceae was evaluated for its possible. The calotropis procera latex had an acidic nature with ph of 4. Evidencebased complementary and alternative medicine. Pdf in vitro antioxidant activity of calotropis procera priya sekar. Antioxidant and antiapoptotic activities of calotropis. Antioxidant potential of a toxic plant calotropis procera r. Pdf antioxidant activity of root of calotropis procera extract was evaluated in a series of in vitro assay involving free radicals. Ethyl acetate extract shows good in vitro antioxidant activity compared to other extract.
Study on antibacterial activity of calotropis procera. Antioxidant activity of crude extract and latex inhibition ranging from 1220mm while latex was found extracted from the calotropis procera more active which showed activity with inhibition zone fig. Antioxidant activity in vitro of calotropis procera extract from arid regions of rajasthan which were then incubated for 30 min. The objective of the present study was to evaluate the antioxidant potential and the wound healing effect of the ethanolic extract of the bark of calotropis procera. The present study intended to investigate the effect of aqueous extract from calotropis procera on the growth of brassica oleracea var botrytis. This shrub is known to possess wide range of pharmacological activity. In the present study antioxidant and antimicrobial activities of extracts from leaves and flowers of local akk calotropis procera were examined. This study verified that the ethanol extract and ethyl acetate fraction from calotropis procera have strong antioxidant activity that were correlated their high level of phenolic content. Free radical scavenging activity of calotropis gigantea on. Antioxidant activity of the leaf extracts of calotropis. Concluding the above findings we state that calotropis procera leaf has optimum antioxidant potential henceforth this plant can be used in future as a valuable.
Antioxidant activity of ethanolic extract of calotropis procera root in wistar rats. Anticancer and antibacterial activity of calotropis procera leaf extract hassan swed alzahrani1, mohamed mutwakil1, jamal sabir 1, kulvinder s. Antioxidant and antimicrobial attributes of different solvent. Phytochemistry and ethnopharmaceutics of calotropis procera oloumi h 3 extensively branched root system and thick leaves with waxy coverage are the xerophytic adaptations. Pdf antioxidant activity of the leaf extracts of calotropis procera. Antioxidant and antibacterial activities of calotropis procera. Ic of 50 the methanol extract of calotropis procera linn. Antifungal, antioxidant and antibacterial activities of calotropis procera mushtaq ahmed, rehmat ali khan, s. In the present study, calotropis procera asclepediaceae was evaluated for its possible hepatoprotective and antioxidant potential. In vitro and in vivo antiproliferative activity of. Pdf efficacy assay of crude leaf extracts of calotropis.
The aim was to evaluate the effect of high molecular weight protein fraction of calotropis procera latex on edema formation and oxidative stress in carrageenaninduced paw inflammation. Evaluation of the antioxidant activity and the healing action. Antioxidant, antimicrobial activities and gcms analysis. Dec 18, 2014 calotropis procera is a medicinal plant used traditionally in animal and human medicine for its digestive and antiasthmatic effects. Phytochemical studies on the fruits, flowers, and leaves of c. Uhplcqtofmsms based profiling of bioactives, antioxidant and antidiabetic potential of leaf extracts and an insight into molecular docking muhammad nadeem 1 muhammad waseem mumtaz 1.
The antioxidant components were extracted using 80% aqueous methanol, 80% aqueous ethanol and 80% aqueous acetone solvents. Antimicrobial activity and phytochemical analysis of calotropis gigantea root, latex extracts. Calotropis procera, latex, antimicrobial, antioxidant, antifungal. Antioxidant activity of root bark of calotropis procera r. The in vitro studies demonstrated the anthelmintic effects p of crude aqueous c ae and crude methanolic extracts c me of calotropis procera flowers on live. Antifungal, antioxidant and antibacterial activities of. Comparative analysis of antioxidant properties of extracts. Antioxidant activity of ethanolic extract of calotropis procera root in.
This present study intended to investigate the effect of aqueous extract from calotropis procera on the growth of brassica oleracea var botrytis. Calotropis gigantea crown flower is a species of calotropis the giant milkweed, calotropis procera, is native to the old world from the arabian peninsula to west, north and east africa, also from southern asia to malaysia. Br asclepiadaceae that can be used against diseases, like diabetes, atherosclerosis, ischeamic heart disease, disorders address for correspondence email. The radical scavenging activity of the aqueous methanol extract and compounds 8 was measured by the 1,1diphenyl2picrylhydrazyl dpph method. Cytotoxic screening of the same compounds was carried out on brine shrimps as well. Antioxidant, antimicrobial activities and gcms analysis of.
The total antioxidant activity was not very pronounced. To study invitro antioxidant activity of calotropis procera. Pdf antioxidant effects of the methanolic, chloroform, hot water and ethyl acetate extracts of the leaves of calotrois procera were tested on the. Ethanol extract of the euphorbia geniculata plant latex had shown almost 93. Evaluation of antihyperglycaemic activity of calotropis. Hepatoprotective and antioxidant activity of root bark of. Shahzaib, abdullah khan, arif waqar zaif, wasim ahmed department of biotechnology, university of science and technology bannu, khyber pakhtunkhwa pakistan key words.
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